When Ladybug/Miraculous Ladybug is in her civilian form she wears twintails, a girly hairstyle that symbolizes femininity and childhood. Miraculous Ladybug is about Marinette growing up and becoming a woman, so the choice of this hairstyle emphasizes her role as a girl in need of growth. This is a very symbolic gesture from the creators of this show, as is her choice to be Ladybug rather than Cat Noir.
In the episode ‘Love Hunter’ Marinette lets her hair down and enjoys herself with Kagami and Adrien. This is the first time that we see her be a joyful young girl free of stress and doubts about her superhero duties. It’s also the first time that Adrien has seen her truly unreserved around him since they met.
This is a very important scene to analyze as it represents a very big change for Marinette. She’s letting her guard down, no longer seeing Kagami and Adrien as competition or someone that she should win over but as genuine friends. She’s enjoying her life without worrying about her heroic obligations, and it’s a great display of her changing character. We’ll continue to follow her journey as she grows and develops into the person she wants to be.